Extraction and Separation of the Bright-Greenish-Yellow Fluorescent Material from Aflatoxigenic Aspergillus spp. Infected Cotton Lint by HPLC-UV/FL
نویسندگان
چکیده
To isolate the bright-greenish-yellow-fluorescence (BGY-F) material associated with aflatoxin contamination on cotton lint, various in vitro chemical and in vivo natural BGY-F reaction products were prepared. BGY-F material was obtained from reactions involving (1) kojic acid (KA) and H2O2, (b) KA, peroxidase, and H2O2, (c) fresh cotton locules treated with KA and H2O2, (d) detached cotton locules inoculated with an aflatoxigenic Aspergillus flavus spore suspension, and (e) live developing cotton bolls inoculated with aflatoxigenic A. flavus. BGY-F preparations separated on TLC resulted in the same Rf value (0.52), indicating the possibility of the same compound. Under HPLC conditions, at a 380 nm wavelength setting, BGY-F retention times ranged 5.74-6.09 min; under fluorescent detection at 435 nm (excitation) and 494 nm (emission), retention times ranged from 5.75 to 6.09 min. Apparently, in all methods used to form the BGY-F compound, both in vivo and in vitro, only one compound with specific chromatographic characteristics was produced, and the product is likely an oxidized form of KA.
منابع مشابه
Molecular identification of aflatoxigenic Aspergillus species in feedstuff samples
Background and Purpose: Aflatoxins are naturally produced by some species of Aspergillus, such as A. flavus and A. parasiticus. Aflatoxins reportedly have carcinogenic effects on human, poultry, and livestock, and therefore could be linked to severe human illnesses. Aflatoxin biosynthesis pathway involves different clustered genes, including structural, regular, and unassigned genes. The presen...
متن کاملCharacterization of aflatoxigenic Aspergillus flavus and A. parasiticus strain isolates from animal feedstuffs in northeastern Iran
Aflatoxins are secondary toxic metabolites produced by some Aspergillus spp. particularly, Aspergillus flavus and A. parasiticus that contaminate food and feed. The objective of this study was to evaluate the contamination of feedstuffs with Aspergillus spp. and detect genes involved in the aflatoxin biosynthesis pathway of A. flavus and A. parasiticus isolates. A total of 110 cow feed samples ...
متن کاملBright greenish-yellow fluorescence and aflatoxin in agricultural commodities.
The corn milling industry has widely accepted the presence of bright greenish-yellow fluorescence under a black light as a presumptive indicator of aflatoxin (a poison produced by the mold Aspergillus flavus). This test was applied to wheat, oats, barley, rice, coconut, white corn, yellow corn, peanuts, sorghum, and soybeans, and evaluated in the laboratory. Our study supported the use of brigh...
متن کاملEvaluation of cotton fibers stickiness by colorimetric method
Stickiness is the physical process of contaminated lint adhering to equipment. The degree of stickiness depends on chemical identity, quantity, and distribution of the sugars. The sugar in cotton fibers may be insect or plant derived. There are several methods for measuring cotton fibers stickiness. In this work, a colorimetric method was used for the measurements. In the method, honeydew was c...
متن کاملCo-inoculation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus flavus to study fungal invasion, colonization, and competition in maize kernels
A currently utilized pre-harvest biocontrol method involves field inoculations with non-aflatoxigenic Aspergillus flavus strains, a tactic shown to strategically suppress native aflatoxin-producing strains and effectively decrease aflatoxin contamination in corn. The present in situ study focuses on tracking the invasion and colonization of an aflatoxigenic A. flavus strain (AF70), labeled with...
متن کامل